Item no. |
S1364-1000 |
Manufacturer |
Selleckchem
|
CASRN |
152044-54-7 |
Amount |
1 g |
Category |
|
Type |
Inhibitors |
Specific against |
other |
ECLASS 10.1 |
32160490 |
ECLASS 11.0 |
32160490 |
UNSPSC |
12000000 |
Alias |
EPO906, Tubulin;Microtubule Associated |
Similar products |
Epothilone |
Available |
|
Administration |
Inject intravenously |
Animal Models |
Mice xenograft model of RPMI 8226 cells |
Cell lines |
KB3-1, KBV-1, Hela, and Hs578T cells |
Chemical Name |
(1S, 3S, 7S, 10R, 11S, 12S, 16R, E)-7, 11-dihydroxy-8, 8, 10, 12, 16-pentamethyl-3-(1-(2-methylthiazol-4-yl)prop-1-en-2-yl)-4, 17-dioxa-bicyclo[14.1.0]heptadecane-5, 9-dione |
Clinical Trials |
A Phase III clinical trial for Epothilone B in patients with ovarian, primary fallopian, or peritoneal cancer has been completed. |
Concentrations |
0–1 uM |
Description |
Epothilone B is a Taxol-like microtubule-stabilizing agent with EC0.01 of 1.8 uM. |
Dosages |
2.5 mg/kg–4 mg/kg |
Formulation |
Dissolved in30% PEG-300 |
IC50 |
1.8 uM (EC0.01) [1], 1.8 uM (EC0.01) [1], 1.8 uM (EC0.01) [1], 1.8 uM (EC0.01) [1], 1.8 uM (EC0.01) [1], 1.8 uM (EC0.01) [1] |
In vitro |
Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 uM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 uM. [2] In MCF-7 cells overexpressing GFP-alpha-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellur level of EpCAM. [5] |
In vivo |
In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6] |
Incubation Time |
72 hours |
Kinase Assay |
Tubulin polymerization assay, Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation oained from three different concentrations. |
Method |
For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis. |
Molecular Weight (MW) |
507, 68 |
Picture ChemicalStructure Description |
Epothilone B (EPO906) Chemical Structure |
Picture Description 1 |
Data independently produced by, , , Dr. Helen Sadik, of Johns Hopkins University, Epothilone B (EPO906)purchased from Selleck, Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay. |
Solubility (25C) |
DMSO 102 mg/mL, Water <1 mg/mL, Ethanol 102 mg/mL |
Storage |
2 years -20CPowder, 2 weeks4Cin DMSO, 2 months-80Cin DMSO |
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