Molecular Biology Guide
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Contents
- Introduction
- Key Principles of Molecular Biology Techniques
- Molecular Cloning Basics (Library Construction)
- cDNA Libraries
- Genomic Libraries
- Polymerase Chain Reaction (PCR)
- PCR Fundamental Principles
- Data Acquisition and Analysis: Gel Electrophoresis
- qRT-PCR Experimental Basics
- Data Acquisition and Analysis: Basic Calculations
- Sample Preparation and Protocols
- Extraction & Purification Protocols
- DNA Extraction & Purification
- RNA Extraction & Purification
- Molecular Cloning: DNA Library Preparation
- PCR Protocols
- Primer Design
- General PCR protocol
- PCR Product Analysis: Gel Electrophoresis
- Reverse transcription protocol
- qRT-PCR
- Troubleshooting Guide
- DNA and RNA extraction
- PCR and qRT-PCR
- FAQs
- Appendix: Fundamentals of Molecular Biology