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Western Blot Application Guide

Western Blot Application Guide

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Everything about the Western Blo method!

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Content

Introduction
Principle

Protein Extraction and Quantitation
Protein Separation: Electrophoresis
Protein Transfer
Membrane Blocking
Primary Antibody Incubation
Secondary Antibody Incubation
Signal Detection
Controls

Recommended Protocol

Sample Preparation
Electrophoresis

Tips for Convincing Your PI to Purchase Pre-Cast Gels
Safety and Good Laboratory Practices
Troubleshooting Guide

High Background
Weak/No Signal
Nonspecific Bands
Diffuse Bands

FAQs
Ordering Information
Antibody Protein Target Index
Contact Information