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ELISA Application Guide

ELISA Application Guide

How to quickly become an ELISA expert?

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Everything about the ELISA method!

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Contents

Introduction
General ELISA Procedure
ELISA Types

Direct ELISA
Indirect ELISA
Sandwich ELISA
Competitive ELISA

ELISA Data Interpretation
Sample Preparation

Cell Culture Supernatants
Cell Extracts
Conditiones Media
Tissue Extract

Recommended Protocols
Troubleshooting Guide

Weak or No Signal
Saturated Signal
High Background
Low Sensitivity
Poor Standard Curve
Poor Replicate Data
Inconsistent Assay-to-Assay Results
Slow Color Development
Plate Imaging Problem

FAQs
Ordering Information
Target Protein Index (By Species)

Human
Rat
Mouse

Contact Information
Appendix: Cell-based ELISA principles & protocols