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RhoA GST Protein: wild type

RhoA GST Protein: wild type

Item no.	RHG01-C
Manufacturer	Cytoskeleton
Amount	8 x 25 ug
Category	Oncology Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton By Organ Heart & Blood Cancer small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. GST-RhoA samples are >90% pure.
Citations	Teckchandani, A. M., Panetti, T. S. and Tsygankov, A. Y. (2005). c-Cbl regulates migration of v-Abl-transformed NIH 3T3 fibroblasts via Rac1. Exp. Cell Res. 307, 247-258. Patil, S. B., Pawar, M. D. and Bitar, K. N. (2004). Phosphorylated HSP27 essential for acetylcholine-induced association of RhoA with PKC&alpha,. Am. J. Physiol. 286, G635-644. Yamashita, T. and Tohyama, M. (2003). The p75 receptor acts as a displacement factor that releases Rho from Rho-GDI. Nat. Neurosci. 6, 461-467. Lesser, C. F. and Miller, S. I. (2001). Expression of microbial virulence proteins in Saccharomyces cerevisiae models mammalian infection. EMBO J. 20, 1840-1849. Slater, S. J., Seiz, J. L., Stagliano, B. A. and Stubbs, C. D. (2001). Interaction of protein kinase C isozymes with Rho GTPases. Biochemistry 40, 4437-4445.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, RhoA, Rho A, RhoA protein, his-RhoA
Similar products	RhoA, Rho A, Small G protein, RhoA protein, his-RhoA
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	22
Product Uses	RhoA biochemistry RhoA GTPase assays RhoA nucleotide exchange assays RhoA binding studies
Material	The human RhoA protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is 52 kDa, consisting of the 24 kDa RhoA protein plus a 28 kDa GST tag in the amino-terminus. For other forms of RhoA as well as many other purified small G-proteins, see our main small G-protein product page.
Biological Activity	The biological activity of RhoA proteins was monitored by measuring the ability of the protein to hydrolyze GTP in the presence and absence of a GTPase-activating protein (RhoGAP) using Cat. # BK055. In the absence of RhoGAP (Cat. #GAS01), GST-RhoA hydrolyzed 0-10% of bound GTP in 5 min at 23CC, while in the presence of RhoGAP (1:1 RhoGAP : GST-RhoA) 75-80% of bound GTP was hydrolyzed under identical conditions.
Figure 1 Legend	Figure 1. GST-RhoA protein purity determination. A 20 ug sample of RHG01 (GST-RhoA molecular weight approx. 52 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

RHG01-C.pdf

RHG01-C-datasheet.pdf

RHG01-C-safety-datasheet.pdf