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Rac1 GST Protein: wild type

Rac1 GST Protein: wild type

Item no.	RCG01-C
Manufacturer	Cytoskeleton
Amount	8 x 25 ug
Category	Oncology Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton By Organ Heart & Blood Cancer small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. GST-Rac1 samples are >90% pure.
Citations	Teckchandani, A. M., Panetti, T. S. and Tsygankov, A. Y. (2005). c-Cbl regulates migration of v-Abl-transformed NIH 3T3 fibroblasts via Rac1. Exp. Cell Res. 307, 247-258. Grabham, P. W., Reznik, B. and Goldberg, D. J. (2003). Microtubule and Rac 1-dependent F-actin in growth cones. J. Cell Sci. 116, 3739-3748. Li, X., Bu, X., Lu, B., Avraham, H., Flavell, R. A. and Lim, B. (2002). The hematopoiesis-specific GTP-binding protein RhoH is GTPase deficient and modulates activities of other Rho GTPases by an inhibitory function. Mol. Cell. Biol. 22, 1158-1171. Lesser, C. F. and Miller, S. I. (2001). Expression of microbial virulence proteins in Saccharomyces cerevisiae models mammalian infection. EMBO J. 20, 1840-1849. Slater, S. J., Seiz, J. L., Stagliano, B. A. and Stubbs, C. D. (2001). Interaction of protein kinase C isozymes with Rho GTPases. Biochemistry 40, 4437-4445.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, Rac1, Rac 1, GST-Rac1, human Rac1
Similar products	Rac1, Rac 1, human Rac1, Small G protein, GST-Rac1
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	22
Product Uses	Rac biochemistry Rac1 GTPase assays Rac1 nucleotide exchange assays Rac1 binding studies
Material	The human Rac1 protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is 50 kDa, consisting of the 22 kDa Rac1 protein plus a 28 kDa GST tag in the amino-terminus. For other forms of Rac1 as well as many other purified small G-proteins, see our main small G-protein product page.
Biological Activity	The biological activity of GST-Rac1 protein was monitored by measuring the ability of the protein to hydrolyze GTP in the presence and absence of a GTPase-activating protein (RhoGAP) using Cat. # BK055. In the absence of RhoGAP (Cat. #GAS01), GST-Rac1 hydrolyzed 40-50% of bound GTP in 5 min at 23CC, while in the presence of RhoGAP (1:1 RhoGAP : GST-Rac1) 75-80% of bound GTP was hydrolyzed under identical conditions.
Figure 1 Legend	Figure 1. GST-Rac1 protein purity determination. A 20 ug sample of RCG01 (GST-Rac1 molecular weight approx. 50 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

RCG01-C.pdf

RCG01-C-datasheet.pdf

RCG01-C-safety-datasheet.pdf