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Rho GAP Protein: catalytic domain GST

Rho GAP Protein: catalytic domain GST

Item no.	GAS01-B
Manufacturer	Cytoskeleton
Amount	4 x 50 ug
Quantity options	1 x 50 ug 4 x 50 ug
Category	Neuroscience Neural Signaling Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Rho Subfamily
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >90% pure.
Citations	Li, X., Bu, X., Lu, B., Avraham, H., Flavell, R. A. and Lim, B. (2002). The hematopoiesis-specific GTP-binding protein RhoH is GTPase deficient and modulates activities of other Rho GTPases by an inhibitory function. Mol. Cell. Biol. 22, 1158-1171.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, p50RhoGAP, Rho GAP, Rho, RhoGAP, GAP, Rho Proteins
Similar products	Rho, GAP, Small G protein, Rho GAP, p50RhoGAP, RhoGAP, Rho Proteins
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	28
Product Uses	In vitro GAP assays with various Rho proteins Inhibition of Rho proteins in vivo by microinjection
Material	p50RhoGAP (sometimes called Cdc42-GAP or Rho GAP) is a GTPase Activating Protein that catalyzes the hydrolysis of GTP for Rac, Rho, and Cdc42 proteins. It appears to be most active for Cdc42 and least active for Rac. The catalytic GAP domain of p50RhoGAP has been purified from a bacterial expression system and is supplied as a GST-fusion protein. This protein is 57 kDa in size and is comprised of the 29 kDa catalytic domain of p50RhoGAP and a 28 kDa GST tag. It is supplied as a lyophilized powder.When reconstituted to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose and 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent cat. # ADV02. p50RhoGAP is also available as a purified GST-tagged full length version (Cat. # GAP01)
Biological Activity	Biological activity of the catalytic domain of p50RhoGAP was verified by the ability of the proteins to enhance GTP hydrolysis for GST-RhoA, GST-Rac1 and GST-Cdc42 proteins (Cat #RHG01 "> RCG01 and CDG01 respectively). At a molar ratio of 1:0.3 (G-protein : catalytic domain of p50RhoGAP) hydrolysis of bound GTP was at least 75% completed after 5 min at room temperature. Hydrolysis of bound GTP in the absence of RhoGAP was 0% (GST-RhoA), 10% (GST-Cdc42) or 45% (GST-Rac1) after 5 min at room temperature. GTPase assays were performed using BK055.
Figure 1 Legend	GST-p50RhoGAP, catalytic domain protein purity determination. A 10 ug sample of GAS01 was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

GAS01-B.pdf

GAS01-B-datasheet.pdf

GAS01-B-safety-datasheet.pdf