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Rho GAP Protein GST

Rho GAP Protein GST

Item no.	GAP01-B
Manufacturer	Cytoskeleton
Amount	4 x 50 ug
Quantity options	1 x 50 ug 4 x 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	other
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are 90% pure.
Citations	Li, X., Bu, X., Lu, B., Avraham, H., Flavell, R. A. and Lim, B. (2002). The hematopoiesis-specific GTP-binding protein RhoH is GTPase deficient and modulates activities of other Rho GTPases by an inhibitory function. Mol. Cell. Biol. 22, 1158-1171.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	p50RhoGAP protein, GAP assays, Rho, rho protein, Cdc42-GAP, Rho GAP
Similar products	Rho, p50RhoGAP protein, GAP assays, rho protein, Cdc42-GAP, Rho GAP
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	28
Product Uses	GAP assays with various Rho proteins Inhibition of Rho proteins in vivo by microinjection
Material	p50RhoGAP (sometimes called Cdc42-GAP or Rho GAP) is a GTPase Activating Protein that catalyzes the hydrolysis of GTP for Rac, Rho, and Cdc42 proteins. It appears to be most active for Cdc42 and least active for Rac. p50RhoGAP has been purified from a bacterial expression system and is supplied as a GST-fusion protein. This protein is 78 kDa in size and is comprised of the 50 kDa full length p50RhoGAP protein and a 28 kDa GST tag. It is supplied as a lyophilized powder.When reconstituted to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose and 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent cat. # ADV02. p50RhoGAP is also available as a purified GST-tagged catalytic (GAP) domain only (Cat. #GAS01)
Biological Activity	Biological activity of full length p50RhoGAP were verified by the ability of the proteins to enhance GTP hydrolysis for GST-RhoA, GST-Rac1 and GST-Cdc42 proteins (Cat #RHG01 RCG01 and CDG01 respectively). At a molar ratio of 1:0.3 (G-protein : full length p50RhoGAP) hydrolysis of bound GTP was at least 75% completed after 5 min at room temperature. Hydrolysis of bound GTP in the absence of RhoGAP was 0% (GST-RhoA), 10% (GST-Cdc42) or 45% (GST-Rac1) after 5 min at room temperature. GTPase assays were performed using BK055.
Figure 1 Legend	Figure 1. GST-p50RhoGAP protein purity determination. A 10 ug sample of GAP01 was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

GAP01-B.pdf

GAP01-B-datasheet.pdf

GAP01-B-safety-datasheet.pdf