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General Actin Buffer

General Actin Buffer

Item no.	BSA01-010
Manufacturer	Cytoskeleton
Amount	1 x 100 ml
Quantity options	1 x 10 ml 1 x 100 ml
Category	Cell Biology Cytoskeleton Reagents & Chemicals Biochemicals Buffers Microfilaments
Type	Buffer
Specific against	other
Citations	Coming soon! For the most recent publications citing this product, please contact our Technical Service department at infohoelzel.de
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12161700
Alias	Actin, Actin Protein, g-actin buffer, general actin buffer, actin buffer
Similar products	Actin, Actin Protein, g-actin buffer, general actin buffer, actin buffer
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: Can the general actin buffer be stored with ATP added or should it be added fresh? Answer 1:On the day of the experiment, the general actin buffer (Cat. #BSA01) should be supplemented with 0.2 mM ATP ((Cat. #BSA04) to create an optimal actin monomer buffer. We do not recommend storing the actin buffer with ATP in it. Prepare the actin buffer + ATP in a volume that is needed for the experiment. Calcium ions and ATP are required for actin conformation. In addition, ATP is hydrolysed during actin polymerization and is required for the polymerization process. Question 2: What is the composition of Cytoskeleton&rsquo, s general actin buffer? Answer 2: Upon resuspension in 100 ml of sterile de-ionized water, the buffer (Cat. #BSA01) will be at 1X strength buffer and contain: 0.2 mM calcium chloride and 5 mM Tris-HCl, pH 8.0.
Weight (grams)	45
Product Uses	- Resuspension of actin protein - Dilution and storage of G-actin protein
Material	This buffer contains 5 mM Tris-HCl pH 8.0 and 0.2 mM CaCl₂. Used as a general G-actin (monomer) buffer with the addition of 0.2 mM ATP (see (Cat. #BSA04) and 0.5 mM DTT (A-buffer). The buffer can be changed to a general F-actin (filament supporting) buffer by the addition of 1/10th volume of actin polymerization buffer (see Cat. # BSA02).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BSA01-010.pdf

BSA01-010-datasheet.pdf

BSA01-010-safety-datasheet.pdf