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Alpha-actinin (rabbit skeletal muscle)

Alpha-actinin (rabbit skeletal muscle)

Item no.	AT01-A
Manufacturer	Cytoskeleton
Amount	2 x 50 ug
Quantity options	2 x 50 ug 10 x 50 ug
Category	Food Safety Allergenes Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton Microfilaments
Type	Proteins
Specific against	Rabbit (Oryctolagus cuniculus)
Purity	Purity is determined by scanning densitometry of the protein on an SDS-PAGE gel. ?-actinin is >90% pure.
Citations	van der Gucht, J., Paluch, E., Plastino, J. and Sykes, C. (2005). Stress release drives symmetry breaking for actin-based movement. Proc. Natl. Acad. Sci. U. S. A. 102, 7847-7852. Lu, S., Carroll, S. L., Herrera, A. H., Ozanne, B. and Horowits, R. (2003). New N-RAP-binding partners &alpha,-actinin, filamin and Krp1 detected by yeast two-hybrid screening: implications for myofibril assembly. J. Cell Sci. 116, 2169-2178. Maul, R. S., Song, Y., Amann, K. J., Gerbin, S. C., Pollard, T. D. and Chang, D. D. (2003). EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 160, 399-407. Karakesisoglou, I., Yang, Y. and Fuchs, E. (2000). An epidermal plakin that integrates actin and microtubule networks at cellular junctions. J. Cell Biol. 149, 195-208. Waterman-Storer, C., Duey, D. Y., Weber, K. L., Keech, J., Cheney, R. E., Salmon, E. D. and Bement, W. M. (2000). Microtubules remodel actomyosin networks in Xenopus egg extracts via two mechanisms of F-actin transport. J. Cell Biol. 150, 361-376.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Actin, Actin Binding Protein, alpha actinin, alpha-actinin, actinin, actinin binding protein,
Similar products	Actin, Actin Binding Protein, alpha actinin, alpha-actinin, actinin, actinin binding protein
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: What is the source of the alpha-actinin Cytoskeleton sells? Answer 1: Cytoskeleton&rsquo, s alpha-actinin (Cat. #AT01) is purified from rabbit skeletal muscle and has a purity of >, 85%. Question 2: Can this alpha-actinin be used for actin bundling experiments? Answer 2: Yes, Cytoskeleton&rsquo, s alpha-actinin (Cat. #AT01) is well-suited for actin bundling experiments using skeletal muscle actin, it will not work with non-muscle isoforms of actin. Briefly, &alpha, -actinin is tested for biological activity by a co-sedimentation assay with Factin. At 2.5 &mu, M skeletal muscle &alpha, -actinin and 25 &mu, M actin in the form of F-actin, >, 70% &alpha, -actinin will co-sediment with F-actin. The reaction is carried out at pH 7.0 for 30 minutes at 24°C (at pH 8.0 &alpha, -actinin will not bind). Spin the mixture at 14, 000 x g for 10 minutes. Probe a western blot with antibodies against alpha-actinin and actin. An SDS-PAGE gel is loaded with supernatant and pellet samples from alpha-actinin + actin and actin alone. After running the gel to separate samples it is stained with coomassie blue and then analyzed for band intensity at 43 and 116 kDal.
Weight (grams)	10
Product Uses	Discover and characterize ?-actinin binding proteins Positive control of actin binding assays Positive control for actin bundling assays
Material	?-actinin has been purified from rabbit skeletal muscle. The protein is supplied as a lyophilized powder and upon reconstitution, will be in the following buffer: 4 mM Tris-HCl pH 7.6, 4 mM NaCl, 20 ?M EDTA, 1% (w/v) sucrose, and 0.2% (w/v) dextran. The lyophilized product is stable at -70C for at least 2 years. AT01 should be resuspended in nanopure water containing 1 mM ?-mercaptoethanol or DTT to the desired working concentration.
Biological Activity	At 2.5 uM (0.25 mg/ml) ?-actinin and 25 uM (1.0 mg/ml) F-actin at pH 7.0 and 24C, approximately 70% of the ?-actinin will co-sediment with F-actin after centrifugation at 150, 000 x g for 1 h.
Figure 1 Legend	?-actinin protein purity determination. 20 ug of AT01 was run on an SDS-PAGE gel and stained with coomassie blue.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

AT01-A.pdf

AT01-A-datasheet.pdf

AT01-A-safety-datasheet.pdf