PUREfrex 2.0

PUREfrex^® 2.0 reconstituted coupled transcription/translation system is a new and improved version of PUREfrex^® 1.0 that debuted in 2011. By improving the purification process for components of PUREfrex^® 2.0RNase, β -galactosidase and LPS contamination has been reduced, the latter to around 0.1EU/µ L of reaction mixture. This, together with a newly optimized composition has boosted protein yield 2-10 times compared to PUREfrex^® 1.0. Further, all proteinaceous components of PUREfrex^® 2.0 are free of fusion tags, allowing users the freedom to incorporate any chosen tag for protein purification/detection.

For proteins whose functional conformation requires the assistance of molecular chaperones or the creation of disulfide bonds, we offer supplemental reagents that work in conjunction with PUREfrex^® 2.0.

Supplements – Molecular Chaperones

These are supplements for PUREfrex^® series reconstituted coupled transcription/translation systems to promote production and solubility of aggregation-prone proteins.

• DnaK Mix (#PF003-0.5-EX)
  


• GroE Mix (#PF004-0.5-EX)
  

Supplements – Forming disulfide bonds

These are supplements for PUREfrex^® series reconstituted coupled transcription/translation systems to promote synthesis of functional proteins containing disulfide bonds.

• DsbC Set (#PF005-0.5-EX)
  


• PDI Set (#PF006-0.5-EX)