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DsbC Set

DsbC Set

Item no.

GFK-PF005-50ML-EX

Manufacturer

Cosmobio

Amount

25 x 2 ml

Quantity options

500 ul Equivalents 5 x 2 ml Equivalents 25 x 2 ml

Specific against

other

Available

Manufacturer - Category

Protein Engineering/Cell Free Synthesis/Reconstituted/Disulfide Bond Supplements

Maker

GFK

Short Description

DsbC Set is a supplement for the PUREfrex® series reconstituted coupled transcription/translation systems, designed to promote synthesis of functional proteins that contain disulfide bonds. \\DsbC Set comprises E.coli disulfide bond isomerase (DsbC), which catalyzes disulfide bridge exchange reactions, and GSSG (oxidized glutathione) to create an oxidizing environment.

Description

Redox Environment Supplements Promote Disulfide Bond Formation
Formation of disulfide bonds is important for folding and stability of secretory proteins such as enzymes or antibodies. Disulfide bonds are usually formed by oxidation of sulfhydryl groups (SH-) of adjacent cysteine residues. Therefore, disulfide bond formation efficiency depends on redox state. Additionally, disulfide bond isomerase which can catalyze the exchange of disulfide bridges may be required for correct cysteine pairing.

Redox Supplement

DsbC Set comprises highly purified E. coli DsbC (a disulfide bond isomerase) which can catalyze disulfide bridge exchange, and GSSG (oxidized Glutathione) to enforce an oxidized environment.

Reagent

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
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