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DnaK Mix

DnaK Mix

Item no.

GFK-PF003-10ML-EX

Manufacturer

Cosmobio

Amount

5 x 2 ml Equivalents

Quantity options

500 ul Equivalents 5 x 2 ml Equivalents 25 x 2 ml

Specific against

other

Available

Manufacturer - Category

Protein Engineering/Cell Free Synthesis/Reconstituted/Folding Supplements

Maker

GFK

Short Description

DnaK Mix is a newly developed supplement for the PUREfrex® series reconstituted coupled transcription/translation systems, designed to promote proper folding and improve solubility for proteins that require molecular chaperones for proper folding. \\It comprises an optimized ratio of highly purified E.coli-derived DnaK, DnaJ and GrpE.

Description

DnaK Mix is a newly developed supplement for the PUREfrex^®series reconstituted coupled transcription/translation systems, designed to promote proper folding and improve solubility for proteins that require molecular chaperones for proper folding. It comprises an optimized ratio of highly purified E.coli-derived DnaK, DnaJ and GrpE.
DnaK Mix is a newly developed supplement for the PUREfrex^®series, designed to assist proper folding and improve solubility of proteins that require molecular chaperones for proper protein folding. DnaK Mix comprises an optimized ratio of highly purified E.coli-derived DnaK, DnaJ and GrpE. ATPase activity of DnaK (Hsp70) is stimulated by co-chaperones DnaJ and GrpE. DnaJ promotes the ATPase activity of DnaK and can bind to hydrophobic regions of synthesized proteins. GrpE stimulates ADP/ATP exchange rate of DnaK.

Chaperone Supplement

DnaK Mix comprises highly purified and ratio-optimized E. coli DnaK, DnaJ and GrpE. DnaK, known as Hsp70, has ATPase activity and is stimulated by co-chaperones DnaJ and GrpE. DnaJ facilitates DnaK ATPase activity and binds to hydrophobic regions of synthesized proteins. GrpE stimulates ADP/ATP exchange of DnaK. DnaK Mix works well with PUREfrex^®series and DsbC Set.

Reagent

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5 x 2 ml Equivalents

available

Delivery expected until 1/17/2025