BARMOTIN/7H6-ANTIGEN (Tight Junction-Associated Protein)

GWB-6BA313

The antibody can be used for Western blotting and immunohistology on frozen sections. Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Several molecules of the tight junction complex have been identified. The monoclonal antibody 7H6 reacts with a 155 kD tight junction-associated protein also designated barmotin. This protein is preferentially localized at the periphery of tight junctions of hepatocytes and other epithelia. The 7H6 antigen is also detectable in the intestine and kidney. Studies with Madin-Darby canine kidney (MDCK) cells indicate the 7H6 antigen to be an important molecule for regulation of the barrier function of tight junctions. Confocal laser scanning microscopy revealed that the expression of 7H6 antigen decreased sequentially during hepatocarcinogenesis. The 7H6 antigen was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern. Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas. but reduced in poorly-differentiated adenocarcinomas. In MDCK cells the disappearance of 7H6 expression is closely related with cell spreading in vitro induced hepatocyte growth factor/scatter factor. This antibody reacts with the human. canine. mouse. rat and chicken protein.

Golgi apparatus (By similarity). Cell junction tight junction (By similarity). Note=Recruited to tight junctions (TJ) during late stages of maturation of the TJ complexes. Excluded from adherens junctions and desmosomes (By similarity).