Neopterin ELISA Kit

Neopterin ELISA Kit Incubation Time: 1 x 1. 5 h 1 x 10 min Standard Range: 1. 35 - 111 nmol/L Specimin / Volumes: 10uL serum plasma urine Substrate / Isotope: TMB 450 nm # of Samples: 1X96 Assays 12 x 6 plate
Intended Use: Enzyme immunoassay for the quantitative determination of neopterin in human serum plasma and urine. Summary and Explanation of Test:Neopterin biosynthesis is closely associated with activation of the cellular immune system. Increased concentrations of neopterin were reported in patients with viral infections suggesting that increased values may originate from the immune response of patients directed against virally infected cells. It was shown that antigenic stimulation of human peripheral blood mononuclear cells leads to neopterin release into cell culture medium and that human macrophages produce neopterin in vitro when stimulated by interferon gamma. The determination of neopterin levels in human body fluids offers a useful and innovative tool to monitor diseases associated with the activation of cell-mediated immunity. Increasing neopterin levels in various infections precede the clinical manifestation and seroconversion. Normally samples are not tested for all possible infections. Therefore the measurement of neopterin in blood donor samples is a useful tool in order to reduce the risk of infections via blood transfusion. Principle of the test: Solid phase enzyme-linked immunosorbent assay (ELISA) based on the basic principle of a competitive ELISA. An unknown amount of antigen in the sample and a fixed amount of enzyme labelled antigen compete for the antibody-binding sites (rabbit-anti-neopterin). Both antigen-antibody complexes bind to the wells of the microtiter strips coated with a goat-anti-rabbit antibody. Unbound antigen is removed by washing. The intensity of the color developed after the substrate incubation is inversely proportional to the amount of antigen in the sample. Results of samples can be determined directly using the standard curve. Storage and Stability: The kit is shipped at ambient temperature and should be stored at 2-8C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the corresponding chapters. The microtiter strips are stable up to the expiry date of the kit in the broken but tightly closed bag when stored at 2-8C.