Hepatitis B Core Ag

IgG2a

Recombinant HBcAg

Monoclonal Antibodies to Infectious Agents and Toxins

10mM PBSApplications Notes : Used in EIA and ImmunoSensor for detection of HBcAg. Each laboratory should determine an optimum. working titer for use in its particular application. Recommended pair for sandwich immunoassay. Capture Detection. 20-511-241371 20-511-241374

Self assemble to form an icosahedral capsid. Most capsid appear to be large particles with a icosahedral symmetry of T=4 and consist of 240 copies of capsid protein through a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Both capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus providing more genome for transcription or bud through the endoplasmic reticulum. Phosphorylation after capsid formation is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex by the importin-(karyopherin-) mediated pathway (By similarity).

Homodimerizes then multimerizes. Interacts with cytosol exposed regions of viral L glycoprotein present in the reticulum-to-Golgi compartment. Interacts with human FLNB (By similarity).

Phosphorylated by host SRPK1 SRPK2 and maybe protein kinase A protein kinase C or GAPDH. Phosphorylation is critical for pregenomic RNA packaging. Protein kinase C phosphorylation is stimulated by HBx protein and may play a role in transport of the viral genome to the nucleus at the late step during viral replication cycle. Protein kinase A phosphorylation may promote capsid assembly (By similarity).