S100A9 (MRP-14)

GWB-44E64F

The monoclonal antibody 1H9 can be used for Western blotting and flow cytometry. Furthermore the monoclonal antibody 1H9 is useful for immuno assays as detector. Use: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recoomended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The monoclonal antibody 1H9 recognizes the KPpL epitope in the C terminal region of the S100A9 (MRP-14) protein. The calcium-binding. migration inhibitory factor-related proteins. MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex. also called MRP-8/MRP-14 complex or calprotectin. comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes. macrophages and ileal tissue eosinophils. In inflammatory conditions small venueles stain with both anti-S100A8 and S100A9. The staining of the two subunits is always coincident. The S100A8/A9 complex has antibacterial. antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. N/AN/A [1] Odink K. G. Clerc R. Cerletti N. Brueggen J. Tarcsay L. Sorg C. and Wiesendanger W. Novel lymphokine related peptides