Item no. |
20-272-193317 |
Manufacturer |
GENWAY
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody |
Applications |
WB, IF, IP |
Clone |
13B3 |
Specific against |
other |
ECLASS 5.1 |
32160702 |
ECLASS 6.1 |
32160702 |
ECLASS 8.0 |
32160702 |
ECLASS 9.0 |
32160702 |
ECLASS 10.0.1 |
32160702 |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
GWB-396CCB |
Similar products |
20-272-193317 |
Available |
|
Genway ID: |
GWB-396CCB |
Clone: |
13B3 |
Isotype: |
IgG1Light chain: kappa |
Immunogen: |
Amino acids 1-425 of Rad50 expressed in E. coli. |
Positive Control: |
Raji |
Target: |
Rad50 |
Localization: |
Nuclear |
Concentration: |
1 mg/ml |
Purification Note: |
From ascitic fluid Storage |
Buffer: |
Phosphate-buffered saline pH 7. 4 containing no preservatives |
Application Note: |
For IP: Use at an assay dependent dilution. Suggested working dilution for 20-272-193317 for western blot with 30 µ g HeLa whole cell extract: 1:1 000-1 500. Immunofluorescence: 1:100-250. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Target protein |
Molecular Weight: |
153 Cellular |
Localization: |
Nuclear Expression system: pGEX Myeloma: NS1 Rad50-13B3 recognizes human Rad50 a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells. |
Function: |
Component of the MRN complex which plays a central role in double-strand break (DSB) repair DNA recombination maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3\' -5\' exonuclease activity which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Cofactor: Binds 1 zinc ion per homodimer (By similarity). |
Subunit: |
Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex at least composed of BRCA1 MSH2 MSH6 MLH1 ATM BLM RAD50 MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. |
Subcellular Location: |
Nucleus. Telomere. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. |
Tissue Specificity: |
Expressed at very low level in most tissues except in testis where it is expressed at higher level. Expressed in fibroblasts. |
Domain: |
The zinc-hook which separates the large intramolecular coiled coil regions contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule thereby forming a V-shaped homodimer. The two heads of the homodimer which constitute the ATP-binding domain interact with the MRE11A homodimer (By similarity). |
Ptm: |
Phosphorylated upon DNA damage probably by ATM or ATR (By similarity). |
Miscellaneous: |
In case of infection by adenovirus E4 the MRN complex is inactivated and degraded by viral oncoproteins thereby preventing concatenation of viral genomes in infected cells. |
Similarity: |
Belongs to the SMC family. RAD50 subfamily. |
Similarity: |
Contains 1 zinc-hook domain. |
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