200kDa Neurofilament heavy [NF-01]

GWB-67AABC

IgG1

Pig

200kDa Neurofilament heavy

> 95 % pure (by PAGE) Storage

ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons and are composed predominantly of three major proteins called NF-L NF-M and NF-H. NF-H is the heavy or high molecular weight neurofilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa with some variation in different species. This monoclonal recognizes specifically the phosphorylated form of NF-H which is normally found only in axons in the adult nervous system. Since closely related phosphorylated epitopes are also found on NF-M this antibody shows some reactivity with phosphorylated forms of this protein also. Interestingly in many damage and disease states the phosphorylated form of NF-H accumulate in the perikarya of neurons. The antibody can be used to identify axons in tissue sections and in tissue culture. It can also be used to study phosphorylated neurofilament accumulations seen in many neurological diseases and following neuronal injury.

There are a number of repeats of the tripeptide K-S-P NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.

Belongs to the intermediate filament family.