RB1 [1F8] (ready-to-use)

GWB-1EDEC4

IgG1Light chain: kappa

Human

RB1

IHC-P: This antibody has been pretitered and ready to use on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 1mM EDTA buffer pH 8. 0 prior to immunostaining. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular

Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated active form of RB1 interacts with E2F1 and represses its transcription activity leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and in particular that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1 SUV420H1 and SUV420H2 leading to epigenetic transcriptional repression. Controls histone H4 \' Lys-20\' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections interactions with SV40 large T antigen HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1\' s activity.

Nucleus.

Phosphorylated in G1 thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen HPV E7 and adenovirus E1A bind to the underphosphorylated active form of pRb.

Defects in RB1 are a cause of bladder cancer [MIM:109800].

Belongs to the retinoblastoma protein (RB) family.