Plasminogen [9E7]

9E7

Full length native protein (purified) (Human).

Plasminogen

Purity tested by electrophoresis. Storage

AP: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Antigen retrieval is not essential but may optimise staining. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Myeloma: x63-Ag8. 653 Plasminogen is a plasma protein synthesised mostly in the liver. It consists of a single polypeptide chain and has a molecular weight of 88. 5 kDa. The plasma concentration is usually in the range of 70 - 200mg/L. Plasminogen is activated by tissue-plasminogen activator urokinase and streptokinase to form plasmin. Activation results from the cleavage and release of the preactivation peptide. Plasmin consists of a heavy (A-) chain which contains 5 kringles and a light (B-) chain with the active site. Plasmin is a trypsin-like protease whose natural substrate is fibrin with binding sites for fibrin residing on the kringles. Plasmin is essential for fibrinolysis and decreased fibrinolytic potential due to congenital defects in plasmin can lead to recurring thrombosis thrombophlebitis and pulmonary embolism.

Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.

Converted into plasmin by plasminogen activators both plasminogen and its activator being bound to fibrin. Cannot be activated with streptokinase.

Secreted.

In the presence of the inhibitor the activation involves only cleavage after Arg-581 yielding two chains held together by two disulfide bonds. In the absence of the inhibitor the activation involves additionally the removal of the activation peptide (By similarity).

In the presence of the inhibitor the activation involves only cleavage after Arg-581 resulting in 2 chains held together by 2 disulfide bonds. Without the inhibitor the activation involves also removal of the activation peptide.

Contains 5 kringle domains.

Contains 1 peptidase S1 domain [view classification].