Item no. |
20-272-190078 |
Manufacturer |
GENWAY
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody |
Applications |
ELISA |
Clone |
M10G4 |
Specific against |
other |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
GWB-31DBB0 |
Similar products |
20-272-190078 |
Available |
|
Genway ID: |
GWB-31DBB0 |
Clone: |
M10G4 |
Isotype: |
IgG |
Immunogen: |
Full length protein (Human). A high molecular weight more than 300 kDa glycoprotein from human milk-fat globule molecule. |
Antigen Species: |
Human |
Specificity: |
Repeat of VNTR extracellular portion of MUC1 molecule. Reacts with VNTR5 and VNTR20?recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1. Does not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate. Belongs to Mab cluster 1-4 according to ISOMB TD-4 classification . No cross-reactivity with egg white avidin. |
Target: |
MUC1 |
Localization: |
Cell Membrane and Cytoplasmic |
Concentration: |
3 mg/ml |
Purification Note: |
From mouse ascitic fluid Storage |
Buffer: |
Phosphate-buffered saline pH 7. 4 containing 0. 1% sodium azide |
Application Note: |
ELISA: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular |
Localization: |
Cell Membrane and Cytoplasmic Myeloma: x63-Ag8. 653 This antibody reacts with MUC1 a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largely consists of a highly conserved O-glycosylated 20 amino acid tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this antibody is the 8-mer SAPDTRPA of the MUC1 tandem repeat as established with epitope fingerprinting. |
Function: |
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. |
Function: |
The beta subunit contains a C-terminal domain which is involved in cell signaling through phosphorylations and protein-protein interactions. Modulates signaling in ERK Src and NF-kappaB pathways. In activated T-cells influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates P53-mediated transcription and determines cell fate in the genotoxic stress response. Binds together with KLF4 the PE21 promoter element of P53 and represses P53 activity. |
Subunit: |
The alpha subunit forms a tight non-covalent heterodimeric complex with the proteolytically-released beta-subunit. Interaction via the tandem repeat region with domain 1 of ICAM1 is implicated in cell migration and metastases. Isoform 1 binds directly the SH2 domain of GRB2 and forms a MUC1/GRB2/SOS1 complex involved in RAS signaling. The cytoplasmic tail (MUC1CT) interacts with several proteins such as SRC CTNNB1 and ERBs. Interaction with the SH2 domain of CSK decreases interaction with GSK3B. Interacts with CTNNB1/beta-catenin and JUP/gamma-catenin and promotes cell adhesion. Interaction with JUP/gamma-catenin is induced by heregulin. Binds PRKCD ERBB2 ERBB3 and ERBB4. Heregulin (HRG) stimulates the interaction with ERBB2 and to a much lesser extent the interaction with ERBB3 and ERBB4. Interacts with P53 in response to DNA damage. Interacts with KLF4. Interacts with estrogen receptor alpha/ESR1 through its DNA-binding domain and stimulates its transcription activity. Binds ADAM17. |
Subcellular Location: |
Apical cell membrane; Single-pass type I membrane protein. Note=Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions. |
Subcellular Location: |
Isoform 5: Secreted. |
Subcellular Location: |
Isoform 7: Secreted. |
Subcellular Location: |
Isoform 9: Secreted. |
Subcellular Location: |
Mucin-1 subunit beta: Cell membrane. Cytoplasm. Nucleus. Note=On EGF and PDGFRB stimulation transported to the nucleus through interaction with CTNNB1 a process which is stimulated by phosphorylation. On HRG stimulation colocalizes with JUP/gamma-catenin at the nucleus. |
Tissue Specificity: |
Expressed on the apical surface of epithelial cells especially of airway passages breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 expressed in tumor cells only. Developmental Stage: During fetal development expressed at low levels in the colonic epithelium from 13 weeks of gestation. |
Ptm: |
Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose 2- and 3-linked galactose 3- and 3 6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3 4-linked GlcNAc. N-glycosylation consists of high-mannose acidic complex-type and hybrid glycans in the secreted form MUC1/SEC and neutral complex-type in the transmembrane form MUC1/TM. |
Ptm: |
Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17. |
Ptm: |
Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane. |
Ptm: |
Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3beta-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1. |
Polymorphism: |
The number of repeats is highly polymorphic. It varies from 21 to 125 in the northern European population. The most frequent alleles contains 41 and 85 repeats. The tandemly repeated icosapeptide underlies polymorphism at three positions: PAPGSTAP[PAQT]AHGVTSAP[DT/ES]R DT -& gt; ES and the single replacements P -& gt; A P -& gt; Q and P-& gt; T. The most frequent replacement DT & gt; ES occurs in up to 50% of the repeats. |
Similarity: |
Contains 1 SEA domain. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.