Transcription factor PU.1

GWB-53D86C

A synthetic peptide corresponding to residues near the N terminus of Transcription factor PU. 1 was used as an immunogen.

WB: 1:5 000 - 20 000IHC: 1:100 - 250FC: 1:70. Transcription factor PU. 1 is a member of the Ets family of transcription factors and is required for the development of multiple hematopoietic lineages (3). It plays a pivotal role in normal myeloid differentiation and regulates the expression of immunoglobulin and other genes that are important for B-cell development. Transcription factor PU. 1 and AML1 a DNA-binding subunit of the CBF transcription factor family act as tumor suppressors in leukemia. The function of PU. 1 is down-regulated by AML1-ETO in myeloid leukemia whereas overexpression of PU. 1 restores normal differentiation (1-2). The lack of transcription factor PU. 1 protein expression in classical Hodgkin\' s disease (cHD) a lymphoproliferative disease of predominantly B-cell origin likely contributes to the lack of immunoglobulin expression and incomplete B-cell phenotype characteristic of the Reed-Sternberg cells in cHD (4). 1. Huang G et al. Nat Genet. 40(1):51-60 20082. Vangala R K et al. Blood 101(1):270-277 20033. Kim H et al. Blood 104(13):3894-3900 20044. Torlakovic E et al. American Journal of Pathology 159:1807-1814 20015. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487