PKR Phospho (pT451)

GWB-FCC770

A synthetic phospho-peptide corresponding to residues surrounding Thr451 of human PKR (Interferon-inducible RNA-dependent protein kinase) was used as immunogen. The antibody only detects PKR phosphorylated on Threonine 446.

WB: 1:1 000-10 000. Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation PKR is autophosphorylated at multiple sites and phosphorylates the subunit of the eukaryotic initiation factor-2 eIF2 (eIF2) inhibiting protein synthesis (1 2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3). 1. Williams B. R. PKR; Oncogene 18: 61122. Cuddihy A. R. et al. Oncogene 18: 26903. Zhang F. et al. . J. Biol. Chem. 276: 249464. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487