Tubulin Beta III (N-term)

GWB-A87042

A synthetic peptide corresponding to residues near the N-term of class III Beta-Tubulin was used as immunogen.

WB: 1:20 000IHC: 1:250 - 500FC: 1:30IP: 1:40. Microtubules are essential parts in eukaryotic cell structures transportation and mitosis and consists mainly of 2 soluble protein subunits alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines breast cancer cell lines and ovarian tumors (3). 1. Wang HW et al. Nature 435: 911-915 2005. 2. Cleveland DW et al. Cell 20: 95-105 1980. 3. Hasegawa S et al. Clin Can Res 9: 2992-2997 2003. 4. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487