UBC-1

GWB-FF50EC

A synthetic peptide corresponding to residues of human UBC1 was used as an immunogen.

WB: 1:1 000 - 10 000IHC: 1:250 - 500ICC: 1:250IP: 1:50. The covalent attachment of ubiquitin to cellular proteins is catalyzed by members of a family of ubiquitin-conjugating enzymes including UBC1 (E2). These enzymes participate in a variety of cellular processes including selective protein degradation DNA repair cell cycle control and sporulation (1). In this mechanism the ATP-coupled activation and subsequent ligation of ubiquitin are catalyzed by separate enzymes (E1 and E3 respectively) functionally linked by ubiquitin carrier protein UBC1 (E2). Carrier protein has been proposed to constitute a family of isozymes having molecular masses of 14 17 20 24 and 32 kDa whose role is to shuttle activated polypeptide in the form of a high-energy thiol ester intermediate to the carboxyl terminus of ubiquitin (2). The order of ubiquitin transfer is from E1 to E2 from E2 to E6-AP and finally from E6-AP to a substrate (3). 1. Seufert W et al. EMBO J. 9(13):4535-41 1990. 2. Haas AL et al. J Biol Chem 263(26):13258-67 1988. 3. Scheffner M et al. Nature 373(6509):81-3 1995. 4. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487