Phospho-Rb (pT373)

GWB-9746E3

A synthetic phospho-peptide corresponding to residues surrounding Thr373 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 373.

WB: 1:500 - 1 000IHC: 1:250 - 500ICC: 1:50 - 100IP: 1:30. Retinoblastoma tumor suppressor protein (Rb) is associated with DNA binding activity and is thought to play a significant role in controlling cell cycle progression during tumor growth (1 2). Cell cycle-dependent phosphorylation by cdk\' s inhibits Rb binding thus allowing cell cycle progression (3). Rb inactivation and cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (4). 1. Lee W. H. et al. The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity. Nature 329: 6422. Sherr C. J. Cancer cell cycles. Science 274: 16723. Knudsen E. S. and J. Y. Wang. Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation. Mol. Cell. Biol. 17: 57714. Lundberg A. S. and R. A. Weinberg. Mol. Cell. Biol. 18 7535. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487