Nck

GWB-9F6EB8

A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.

WB: 1:500-10 000IHC: 1:100-250ICC: 1:250-500FC: 1:60IP: 1:50. Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally Nck has been shown to associate with BLNK PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2). 1. W Li P Hu EY Skolnik A Ullrich and J Schlessinge; Mol. Cell. Biol. 12 1992 5824-5833 Vol 12 No. 122. M M Chou J E Fajardo and H Hanafusa; Mol Cell Biol. 1992 December; 12(12): 58343. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487