Caldesmon (S789)

GWB-E63B70

A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon was used as immunogen.

WB: 1:500IHC: 1:250ICC: 1:250FC: 1:20IP: 1:80. Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin myosin tropomyosin and calmodulin (1 2). Smooth muscle caldesmon is an elongated molecule with a calmodulin tropomyosin and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4). 1. Humphrey M. B. et al. Cloning of cDNAs encoding human caldesmons. Gene 112: 1972. Bryan J. et al. Cloning and expression of a smooth muscle caldesmon. J Biol Chem. 264: 138733. Bryan J. Caldesmon: fragments sequence and domain mapping. Ann N Y Acad Sci. 599: 1004. Fraser I. D. et al. The inhibitory complex of smooth muscle caldesmon with actin and tropomyosin involves three interacting segments of the C-terminal domain 4. Biochemistry 36: 54835. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487