Bcl-x

GWB-8BE3E3

A synthetic peptide corresponding to residues between BH3 and BH3 of Human Bcl-x was used as the immunogen. This antibody should recognize Bcl-xL Bcl-xS and Bcl-x(beta) as the immunogen sequence is common to them. The antibody does not cross-react with o

WB: 1:500 - 1000IHC: 1:50 - 1:100ICC: 1:100FC: 1:50IP: 1:10. Bcl-x a member of the Bcl-2 protein family inhibits cell death or apoptosis (1). Bcl-x is expressed as two isomeric forms Bcl-xL and Bcl-xS and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins including Bax Bak and Bcl-2(2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis. 1. Kornasio R Riederer I Butler-Browne G et al Î ² -hydroxy-Î ² -methylbutyrate (HMB) stimulates myogenic cell proliferation differentiation and survival via the MAPK/ERK and PI3K/Akt pathways Biochim Biophys Acta. 1793 (2009) 755-763[Application: ICC]2. Ge F Lu XP Zeng HL et al Proteomic and Functional Analyses Reveal a Dual Molecular Mechanism Underlying Arsenic-Induced Apoptosis in Human Multiple Myeloma Cells J. Proteome Res. 8 (2009) 3006-3019[Application: WB]3. Xie W Song CY Young NL et al Histone H3 Lysine 56 Acetylation Is Linked to the Core Transcriptional Network in Human Embryonic Stem Cells Molecular Cell 33 (2009) 417-427[Application: WB]4. Scholl FA Dumesic PA Barragan DI et al Mek1/2 MAPK Kinases Are Essential for Mammalian Development Homeostasis and Raf-Induced Hyperplasia Developmental Cell 12 (2007) 615-629[Application: IHC]5. Kaushansky N Altmann DM Ascough S et al HLA-DQB1*0602 Determines Disease Susceptibility in a New Humanized Multiple Sclerosis Model in HLA-DR15 (DRB1*1501; DQB1*0602) Transgenic Mice J. Immunol. 183 (2009) 3531 - 3541[Application: ]6. Tan KB Lee YS Immunoexpression of Bcl-x in squamous cell carcinoma and keratoacanthoma: differences in pattern and correlation with pathobiology Histopathology 55 (2009) 338-345[Application: IHC]7. Kornasio R Riederer I Butler-Browne G et al Î ² -hydroxy-Î ² -methylbutyrate (HMB) stimulates myogenic cell proliferation differentiation and survival via the MAPK/ERK and PI3K/Akt pathways Biochim Biophys Acta. 1793 (2009) 755-763[Application: ICC]8. Gallenne T Gautier F Oliver L et al Bax activation by the BH3-only protein Puma promotes cell dependence on antiapoptotic Bcl-2 family members J. Cell Biol. 185 (2009) 279 - 290[Application: IP]9. Ge F Lu XP Zeng HL et al Proteomic and Functional Analyses Reveal a Dual Molecular Mechanism Underlying Arsenic-Induced Apoptosis in Human Multiple Myeloma Cells J. Proteome Res. 8 (2009) 3006-3019[Application: WB]10. Gallenne T Gautier F Oliver L et al Bcl-x J Cell Biol 185 (2009) 279-290[Application: IP]11. Priault M Hue E Marhuenda F et al Differential Dependence on Beclin 1 for the Regulation of Pro-Survival Autophagy by Bcl-2 and Bcl-xL in HCT116 Colorectal Cancer Cells PLoS One 5 (2010) e8755[Application: IP]1. Boise L. H. et al. Cell 74(4): 597-608 (1993)2. Sedlak T. W. et al. PNAS U S A. 92(17): 7834-8 (1995)3. Cheng E. H. et al. Nature 379(6565): 554-6 (1996)4. Minn A. J. et al. EMBO. J. 18: 632-643 (1999)5. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487