RAD17

RAD17

Phosphorylated synthetic peptide corresponding to a region within human RAD17 (locus link ID 5884) around serine 645.

Antisera to human phosphorylated RAD17 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Western Blotting - 1:500 - 1:5000 Summary: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17 a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC) and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene which encode four distinct proteins have been reported. Summary: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17 a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC) and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene which encode four distinct proteins have been reported. Two pseudogenes located on chromosomes 7 and 13 have been identified. [provided by RefSeq]. 1. Post. S. et al. (2001) Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G1/S checkpoint activation in response to DNA damage.