Item no. |
18-511-244849 |
Manufacturer |
GENWAY
|
Amount |
0,1 mg |
Category |
|
Type |
Antibody |
Applications |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
GWB-AF382F |
Similar products |
18-511-244849 |
Available |
|
Genway ID: |
GWB-AF382F |
Host Animal: |
Rabbit |
Immunogen: |
Synthetic peptide corresponding to 14 amino acids near the amino terminus of human PAK2 (Genbank accession No. NP_002568) |
Specificity: |
p21 Activated Kinase 2 (PAK2) N-terminal |
Specificity: |
Reacts to the N-terminal of human mouse and rat PAK2. The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac (1). Of the six PAK proteins only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment(2). |
Type of Product: |
Polyclonal Antibodies to Enzymes |
Concentration: |
Lot specificPreservatives: NaN3 |
Buffer: |
PBSApplications Notes : Suitable for use in Western blot (0. 5?1ug/ml). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. |
Warning: |
This product contains sodium azide which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0. 1â ??1. 0%. When disposing of this reagent through lead or copper plumbing flush with copious volumes of water to prevent azide build-up in drains. Rabbit anti PAK2 (NT). Rabbit Antibody to Human p21-activated kinase 2 (PAK2) N-terminal |
Function: |
The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6 histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is at least in part mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. |
Catalytic |
Activity: |
ATP + a protein = ADP + a phosphoprotein. |
Enzyme Regulation: |
Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer enables phosphorylation of Thr-402 and allows the kinase domain to adopt an active structure (By similarity). Following caspase cleavage autophosphorylted PAK-2p34 is constitutively active. |
Subunit: |
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with SH3MD4. Interacts with and activated by HIV-1 Nef. PAK-2p34 interacts with ARHGAP10. |
Subcellular Location: |
Serine/threonine-protein kinase PAK 2: Cytoplasm. |
Subcellular Location: |
PAK-2p34: Nucleus. Cytoplasm perinuclear region. Membrane; Lipid-anchor. Note=Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. |
Tissue Specificity: |
Ubiquitously expressed. Higher levels seen in skeletal muscle ovary thymus and spleen. |
Ptm: |
Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage both peptides PAK-2p27 and PAK-2p34 become highly autophosphorylated with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402 because PAK-2p27 is acting as an exogenous substrate. |
Ptm: |
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34. |
Ptm: |
Ubiquitinated leading to its proteosomal degradation. |
Ptm: |
PAK-2p34 is myristoylated. |
Similarity: |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. |
Similarity: |
Contains 1 CRIB domain. |
Similarity: |
Contains 1 protein kinase domain. |
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