Rabbit anti Collagen Type IV

Rabbit

Collagen Type IV

Polyclonal Antibodies to Collagens and Extracellular Matrix Proteins

0. 125M Sodium borate 0. 075M Sodium chloride 0. 005M EDTA pH 8. 0Applications Notes : Has been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve and for non-denaturing immunoblot. Product was assayed by immunoblot (non-denaturing) and found to be reactive against Collagen IV at a dilution of 1:5 000 to 1:10 000. This product was also assayed against 1. 0ug of Collagen IV in a standard ELISA using Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG (H& L) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4 000 to 1:8 000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Type IV collagen is the major structural component of glomerular basement membranes (GBM) forming a \' chicken-wire\' meshwork together with laminins proteoglycans and entactin/nidogen. Potently inhibits endothelial cell proliferation and angiogenesis. Inhibits angiogenesis potentially via mechanisms involving cell surface proteoglycans and the alpha and beta integrins of endothelial cells.

Secreted extracellular space extracellular matrix basement membrane.

Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix) and a short N-terminal triple-helical 7S domain.

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

The trimeric structure of the NC1 domains may be stabilized by covalent bonds between Lys and Met residues.

Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.

Belongs to the type IV collagen family.