MDC

IgG

Rat

Secreted

0. 1% Sodium Azide; Constituents: PBS

Secreted MDC (macrophage-derived chemokine) is a 69-amino acid CC chemokine with a NH2-terminalsequence of 24 residues as a signal sequence. It was originally cloned from human monocyte-derived macrophages (ref 1). Recombinant MDC attracts monocyte-derived dendritic cells activated lymphocytes and natural killer cells (ref 1 2). MDC has anti-HIV-1 activity (ref 2). Expression of MDC mRNA in macrophages is enhanced by LPS IL-1 and TNF (ref 3). MDC is a functional ligand for CCR4 (ref 4).

Interacts with several proteins involved in the DNA damage response although not all these interactions may be direct. Interacts with H2AFX which requires phosphorylation of H2AFX on \' Ser-139\' . Interacts with the MRN complex composed of MRE11A/MRE11 RAD50 and NBN. Interacts with CHEK2/CHK2/CDS1 which requires ATM-mediated phosphorylation of \' Thr-68\' within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex SMC1A and TP53BP1. Interacts with ATM and FANCD2 and these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex composed of G22P1/KU70 XRCC5/KU80 and PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation which is essential for DNA double strand break (DSB) repair. When phosphorylated by ATM interacts with RNF8.

Highly expressed in testis.

Phosphorylated upon exposure to ionizing radiation (IR) ultraviolet radiation (UV) and hydroxyurea (HU). Phosphorylation in response to IR requires ATM NBN and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.

Contains 1 FHA domain.