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Hsp27 phospho S82

Hsp27 phospho S82

Item no.	18-272-196648
Manufacturer	GENWAY
Amount	0.05 ml
Category	Antibodies Western Blot Primary Antibodies
Type	Antibody
Applications	WB
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-39FD5E
Similar products	18-272-196648
Available
Genway ID:	GWB-39FD5E
Isotype:	IgG
Immunogen:	Synthetic phospho peptide (Human).
Antigen Species:	Human
Specificity:	When tested by Western blotting on TNF alpha stimulated HeLa lysates the antibody detects a single clean band of about 27kD. This band is only blocked by the phospho peptide immunogen and not by an equivalent non-phospho peptide or by a generic phospho peptide. This confirms that the antibody is specific for phospho S82 of Hsp27.
Positive Control:	HeLa cells treated with TNF alpha
Target:	Hsp27
Purification Note:	The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated HSP27. Storage
Preservative:	0. 05% Sodium Azide. Constituents: 50% Glycerol PBS 1mg/ml BSA. pH 7. 3
Application Note:	WB: 1/1000. Detects a band of approximately 27 kDa (predicted
Molecular Weight:	27 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments and stimulates HSP27 binding to the IKK complex.
Function:	Involved in stress resistance and actin organization.
Subunit:	Associates with alpha- and beta-tubulin microtubules and CRYAB. Interacts with HSPB8 (By similarity). Interacts with HSPBAP1 and TGFB1I1.
Similarity:	Belongs to the small heat shock protein (HSP20) family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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