ADAM15

Manufacturer	GENWAY
Category	Oncology Antibodies Western Blot Primary Antibodies By Organ Skin Cancer
Type	Antibody
Specific against	other
Applications	WB
Amount	0.025 mg
Item no.	18-272-195434
eClass 6.1	32160702
eClass 9.0	32160702
Available
Alias	GWB-11C883
Genway ID:	GWB-11C883
Isotype:	IgG
Immunogen:	Synthetic peptide corresponding to the propeptide domain (Human).
Antigen Species:	Human
Target:	ADAM15
Localization:	Type I membrane protein.
Concentration:	1 mg/ml Storage
Preservative:	0. 05% Sodium Azide; Constituents: 50% Glycerol PBS
Application Note:	WB: Use at a dilution of 1/1000. This concentration is determined for tissue or cell lysates using an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. A starting dilution of 1/5000 of the antibody is recommended for chemiluminescent substrates. Detects a band of approximately 110 kDa zymogen) but not the 90 kDa (furin-processed form). A series of cleaved products at 55 kDa 48 kDa and 40 kDa can also be seen in reduced immunoblots of cell lysates. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular
Localization:	Type I membrane protein. ADAM15 is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain a disintegrin-like domain as well as a EGF-like domain. Through its disintegrin-like domain this protein specifically interacts with the integrin beta chain beta 3 (ITGB3). It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Function:	May be involved in cell-surface proteolysis cell adhesion or intracellular protein maturation. Cofactor: Binds 1 zinc ion per subunit (By similarity).
Subunit:	Interacts with ITAGV-ITGB3 (vitronectin receptor) PACSIN3 and SNX9. PACSIN3 and SNX9 preferentially bind the precursor but not the processed form of ADAM15 suggesting that the interaction occurs in a secretory pathway compartment prior to the medial Golgi (By similarity). Interacts specifically with Src family protein-tyrosine kinases (PTKs). Interacts with SH3PXD2A.
Subcellular Location:	Membrane; Single-pass type I membrane protein.
Tissue Specificity:	Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes.
Domain:	The cytoplasmic domain interacts with endophilin I and sorting nexin 9 (By similarity).
Domain:	Desintegrin domain binds to integrin alphaV-beta3.
Domain:	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Ptm:	The precursor is cleaved by a furin endopeptidase (By similarity).
Ptm:	Phosphorylation increases association with PTKs.
Similarity:	Contains 1 disintegrin domain.
Similarity:	Contains 1 EGF-like domain.
Similarity:	Contains 1 peptidase M12B domain [view classification].

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 0.025 mg

available

Delivery expected until 7/26/2024