Hamster Anti-Mouse CD154

Armenian Hamster IgG kappa

Activated mouse Th1 clone D1. 6 1

CD154 (Mouse CD40 ligand/gp39) Mr 39-kDa CD154 formerly known as CD40 ligand and gp39 is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. 1-7 It is an important accessory molecule in T cell-B cell costimulatory interactions and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0 Th1 and Th2 T cell clones. Its expression is transient and cyclosporinsensitive. 6 The MR1 monoclonal antibody binds to murine CD154 with high affinity blocks binding to CD40 and blocks CD154 function. 1 5 Administration of this antibody to mice blocks the ability to mount primary and secondary immune responses to TD antigens yet does not alter the immune response to TI antigens. 4.

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin eyes or clothing. Wear eye or face protection when handling. If skin or eye contact occurs wash with copious amounts of water. If ingested or inhaled contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. 1. Noelle R. J. M. Roy D. M. Shepherd I Stamenkovic J. A. Ledbetter and A. Aruffo. 1992. Proc. Natl. Acad. Sci. U. S. A. 89:6550. 2. Aruffo A. M. Farrington D. Hollenbaugh X. Li A. Milatovich S. Nonoyama J. Bajorath L. S. Grosmaire R. Stenkamp and M. Neubauer. 1993. Cell 72:291. 3. Hollenbaugh D. L. S. Grosmaire C. D. Kullas N. J. Chalupny S. Braesch-Andersen R. J. Noelle I. Stamenkovic J. A. Ledbetter and A. Aruffo. 1992. EMBO J. 11:4313. 4. Foy TM. DM Shepherd F. H. Durie A. Aruffo J. A. Ledbeter and R. J. Noelle. 1993. J. Exp. Med. 178:1567. 5. Foy T. M. D. M. page T. J. Waldschmidt A. Schoneveld J. D. Laman S. R. Masters L. Tygrett J. A. Ledbetter A. Aruffo and E. Claassen. 1995. J. Exp. Med. 182:1377. 6. Roy M. T. J. Waldschmidt A. Aruffo J. A. Ledbetter and R. J. Noelle. 1993. J. Immunol. 151:2497. 7. Armitage R. J. W. C. Fanslow L. Stockbine T. A. Sato K. N. Clifford B. M. Macduff D. M. Anderson S. D. Gimpel T. Davis-Smith and C. R. Maliszewski. 1992. Nature 357:80.