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Beta-Defensin 3 (BD-3), Rat

Beta-Defensin 3 (BD-3), Rat

Item no.	Z02947-1
Manufacturer	GenScript
Amount	1mg
Category	Neuroscience Neuroinflammation Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton Intermediate filaments
Type	Proteins
Format	Sterile Filtered White lyophilized (freeze-dried) powder.
Specific against	Rat
Purity	>95% by SDS-PAGE and HPLC analyses.
Sequence	KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02947_1-Beta_Defensin_3_BD_3_Rat, Beta defensin-3, also known as BD-3 and DEFB-3, is a membrane active cationic peptide that functions in inflammation and innate immune responses and coded by Defb 3 gene on chromosome 8 in mouse. There are at least 30 beta-defensins which are distinguished from alpha-defensins by the connectivity pattern of their three intramolecular disulfide bonds. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 productions and inhibits TIMP1 and 2 expressions.</td></tr><tr><th>M.W.</th><td colspan="7"> Approximately 4.5 kDa, a single non-glycosylated polypeptide chain containing 41 amino acid residues.</td></tr><tr><th>Purity</th><td colspan="7"> >95% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 1EU/ug of rRtBD-3 as determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> Fully biologically active when compared to standard. Measured by its antimicrobial activity against, E. coli . The ED50 for this effect is typically 4-20ug/ml.</td></tr><tr><th>Storage</th><td colspan="7"> This lyophilized preparation is stable at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -70C. Avoid repeated freeze/thaw cycles. </td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.</td></tr><tr><th>Reconstitution</th><td colspan="7"> We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <=-20C. Further dilutions should be made in appropriate buffered solutions.</td></tr><tr><th>Physical Appearance</th><td colspan="7"> Sterile Filtered White lyophilized (freeze-dried) powder.</td></tr><tr><th>Sequence</th><td colspan="7"> KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K</td></tr>
Similar products	Beta-Defensin
Available
Specificity	Fully biologically active when compared to standard. Measured by its antimicrobial activity against, E. coli . The ED50 for this effect is typically 4-20ug/ml.
Specificity	Fully biologically active when compared to standard. Measured by its antimicrobial activity against, E. coli . The ED50 for this effect is typically 4-20ug/ml.
Description	Beta defensin-3, also known as BD-3 and DEFB-3, is a membrane active cationic peptide that functions in inflammation and innate immune responses and coded by Defb 3 gene on chromosome 8 in mouse. There are at least 30 beta-defensins which are distinguished from alpha-defensins by the connectivity pattern of their three intramolecular disulfide bonds. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 productions and inhibits TIMP1 and 2 expressions.
Endotoxin Level	Less than 1EU/ug of rRtBD-3 as determined by LAL method.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
M.W.	Approximately 4.5 kDa, a single non-glycosylated polypeptide chain containing 41 amino acid residues.
Product Line	Cytokine, Chemokines & Growth Factors
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <=-20C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -70C. Avoid repeated freeze/thaw cycles.
Product Origin	USA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1mg

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