Comparison

GenCrispr Cas9-N-NLS Nuclease European Partner

Item no. Z03388-100
Manufacturer GenScript
Amount 100 ug (4mg/ml)
Category
Type Proteins
Specific against other
Dry ice Yes
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Available
Description
GenCrispr Cas9-N-NLS nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein with a N-terminal nucleic localization signal (NLS) that can be used for genome editing by inducing site-specific DNA double stranded breaks. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the NLS. Compared with the mRNA or plasmid systems, transcription and translation processes are not required. This DNA-free system avoids the risk of inserting foreign DNA into the genome, which can be quite useful for gene editing-based disease therapy. Our highly pure and active Cas9 nuclease meets all of the researcher´ s requirements (e.g. in vitro cleavage assay, RNP complex transfection, micro injection). Product Source: GenCrispr Cas9-N-NLS is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with a N-terminal nuclear localization signal (NLS). Key features: .DNA-free: no external DNA added to system. High cleavage efficiency: NLS ensures the entry of Cas9 protein into nuclei. Low off target: transient expression of Cas9 nuclease. Time-saving: no need for transcription and translation.With this Cas9 Nuclease, you can: Screening the highly efficient and specific targeting gRNAs using in vitro DNA cleavage. In vivo gene editing combined with specific gRNA by electroporation or injection.
Shipping Temperature
4C
Storage Temperature
-20C
Manufacturers Category
Molecular Biology
Manufacturers Product Line
Genome Editing
Product Origin
USA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug (4mg/ml)
Available: In stock
available

Delivery expected until 1/10/2025 

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close