Item no. |
Z02974-10 |
Manufacturer |
GenScript
|
Amount |
10 ug |
Category |
|
Type |
Proteins |
Specific against |
Human |
Purity |
> 95% by SDS-PAGE and HPLC analyses. |
Sequence |
Thr27-Pro200 (accession number:Q8N4W3), expressed with an N-terminal Met. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
protein/Z02974_10-G_CSF_Human,Granulocyte Colony-Stimulating Factor (G-CSF), contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17. Recombinant, human Granulocyte Colony-Stimulating Factor (rhG-CSF), produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE.</td></tr><tr><th>M.W.</th><td colspan="7"> 18.8kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <0.2 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50, 1.0× 107 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant, human Granulocyte Colony-Stimulating Factor (rhG-CSF), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4C or up to 3 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against 25mM Tris, pH8.0.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Thr27-Pro200 (accession number:Q8N4W3), expressed with an N-terminal Met.</td></tr><tr><th>Note</th><td colspan="7"> For research use only</td></tr> |
Similar products |
G-CSF |
Available |
|
Specificity |
ED50, 1.0x 107 IU/mg. |
Specificity |
ED50, 1.0x 107 IU/mg. |
Description |
Granulocyte Colony-Stimulating Factor (G-CSF), contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17. Recombinant, human Granulocyte Colony-Stimulating Factor (rhG-CSF), produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE. |
Endotoxin Level |
<0.2 EU/ug, determined by LAL method. |
Formulation |
Lyophilized after extensive dialysis against 25mM Tris, pH8.0. |
M.W. |
18.8kDa, observed by reducing SDS-PAGE. |
Note |
For research use only |
Product Line |
Cytokine, Chemokines & Growth Factors |
Reconstitution |
Reconstituted in ddH2O at 100 ug/ml. |
Storage |
Lyophilized recombinant, human Granulocyte Colony-Stimulating Factor (rhG-CSF), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4C or up to 3 months at -20C. |
Manufacturers Category |
Proteins |
Manufacturers Product Line |
Growth Factors |
Product Origin |
USA |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.