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TNF-α, Human

TNF-α, Human

Item no.	Z01001-1
Manufacturer	GenScript
Amount	1 mg
Category	Oncology Food Safety Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience By Cell Type Carcinoma Hematology Leukemia Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Cardiovascular Research Ischemia & Reperfusion Injury Contaminants Metals By Organ Lung Cancer Progenitor Markers
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	Greater than 98% as determined by: (a) Analysis by SEC-HPLC, (b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel
Sequence	MVRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
Citations	Angelini DJ, et al . TNF{alpha} Increases Tyrosine Phosphorylation of Vascular Endothelial-Cadherin and Opens the Paracellular Pathway Through Fyn Activation in Human Lung Endothelia. Am. J. Physiol. Lung Cell Mol. Physiol. Aug 2006. Angelone DF, et al . Innate Immunity of the Human Newborn Is Polarized Toward a High Ratio of IL-6/TNF-{alpha} Production In Vitro and In Vivo. Pediatr. Res. Jun 2006. Frankel SK, et al . TNF-alpha sensitizes normal and fibrotic human lung fibroblasts to Fas-induced apoptosis. Am. J. Respir. Cell Mol. Biol. Mar 2006, 34(3): 293-304.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z01001_1-Tumor_Necrosis_Factor_alpha_TNF_alpha_Human,Tumor Necrosis Factor-alpha (TNF-a), is a homotrimer with a subunit molecular mass of 17,000 Da. Tumor Necrosis Factor-alpha (TNF-a) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases, and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. Recombinant human tumor necrosis factor-alpha (TNF-a), is a non-glycosylated cytokine produced from, E. coli, using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17,484 Da. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.</td></tr><tr><th>M.W.</th><td colspan="7"> 17,300 Da+/-10% determined by reduced SDS-PAGE</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 98% as determined by: (a) Analysis by SEC-HPLC, (b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (1 IEU/ug) determined by LAL test</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 3.0×107 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized samples are stable for up to 12 months from date of delivery if stored between -20C and -70C. Avoid repeated freeze-thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against PBS, pH7.3.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended that the lyophilized, recombinant human tumor necrosis factor alpha (TNF-alpha), be reconstituted in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Sequence</th><td colspan="7"> MVRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL </td></tr><tr><th>Reference</th><td colspan="7">Angelini DJ, et al . TNF{alpha} Increases Tyrosine Phosphorylation of Vascular Endothelial-Cadherin and Opens the Paracellular Pathway Through Fyn Activation in Human Lung Endothelia. Am. J. Physiol. Lung Cell Mol. Physiol. Aug 2006. Angelone DF, et al . Innate Immunity of the Human Newborn Is Polarized Toward a High Ratio of IL-6/TNF-{alpha} Production In Vitro and In Vivo. Pediatr. Res. Jun 2006. Frankel SK, et al . TNF-alpha sensitizes normal and fibrotic human lung fibroblasts to Fas-induced apoptosis. Am. J. Respir. Cell Mol. Biol. Mar 2006, 34(3): 293-304.</td></tr>
Similar products	Tumor
Available
Specificity	The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 3.0x107 IU/mg.
Specificity	The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 3.0x107 IU/mg.
Description	Tumor Necrosis Factor-alpha (TNF-a), is a homotrimer with a subunit molecular mass of 17, 000 Da. Tumor Necrosis Factor-alpha (TNF-a) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases, and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. Recombinant human tumor necrosis factor-alpha (TNF-a), is a non-glycosylated cytokine produced from, E. coli, using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17, 484 Da. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Endotoxin Level	Less than 0.1 ng/ug (1 IEU/ug) determined by LAL test
Formulation	The protein was lyophilized after extensive dialysis against PBS, pH7.3.
M.W.	17, 300 Da+/-10% determined by reduced SDS-PAGE
Product Line	Cytokine, Chemokines & Growth Factors
Reconstitution	It is recommended that the lyophilized, recombinant human tumor necrosis factor alpha (TNF-alpha), be reconstituted in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage	Lyophilized samples are stable for up to 12 months from date of delivery if stored between -20C and -70C. Avoid repeated freeze-thaw cycles.
Manufacturers Category	Proteins
Manufacturers Product Line	Cytokines
Product Origin	USA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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