Item no. |
Z00377-1 |
Manufacturer |
GenScript
|
Amount |
1 mg |
Category |
|
Type |
Proteins |
Specific against |
Human (Homo sapiens) |
Purity |
Greater than 98.0% as determined by: (a)Analysis by RP-HPLC (b)Anion-exchange FPLC (c)Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
Sequence |
MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS |
Citations |
Suzuki A, et al . Interleukin-15 increases hepatic regenerative activity. J. Hepatol. Sep 2006, 45 (3): 410-418. Lin SJ, et al . Effect of interleukin-15 on alloreactivity in umbilical cord blood. Transpl. Immunol. Aug 2006, 16 (2): 112-116. Waldmann TA. The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design. Nat. Rev. Immunol. Aug 2006, 6 (8): 595-601. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
protein/Z00377_1-Interleukin_15_IL_15_Human,Interleukin 15 (IL-15), is a cytokine that possesses a variety of biological functions. Interleukin 15 (IL-15) stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R. Recombinant human Interleukin 15 (IL-15), produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12,774 Da.</td></tr><tr><th>M.W.</th><td colspan="7"> 12,774 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 98.0% as determined by: (a)Analysis by RP-HPLC (b)Anion-exchange FPLC (c)Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (1 EU/ug) of recombinant human Interleukin-15 (IL-15) as determined by LAL test.</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be <0.5 ng/ml, corresponding to a Specific Activity of 2x106 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, recombinant human Interleukin 15 (IL-15), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin 15 (IL-15) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7">Recombinant human Interleukin-15 (IL-15), was lyophilized after extensive dialysis against 20mM PB, pH7.5, 150mM NaCl buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized, Recombinant Human Interleukin 15 (IL-15), in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Sequence</th><td colspan="7"> MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS </td></tr><tr><th>Reference</th><td colspan="7">Suzuki A, et al . Interleukin-15 increases hepatic regenerative activity. J. Hepatol. Sep 2006, 45 (3): 410-418. Lin SJ, et al . Effect of interleukin-15 on alloreactivity in umbilical cord blood. Transpl. Immunol. Aug 2006, 16 (2): 112-116. Waldmann TA. The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design. Nat. Rev. Immunol. Aug 2006, 6 (8): 595-601.</td></tr> |
Similar products |
Interleukin-15 |
Available |
|
Specificity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be <0.5 ng/ml, corresponding to a Specific Activity of 2x106 IU/mg. |
Specificity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be <0.5 ng/ml, corresponding to a Specific Activity of 2x106 IU/mg. |
Description |
Interleukin 15 (IL-15), is a cytokine that possesses a variety of biological functions. Interleukin 15 (IL-15) stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R. Recombinant human Interleukin 15 (IL-15), produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12, 774 Da. |
Endotoxin Level |
Less than 0.1 ng/ug (1 EU/ug) of recombinant human Interleukin-15 (IL-15) as determined by LAL test. |
Formulation |
Recombinant human Interleukin-15 (IL-15), was lyophilized after extensive dialysis against 20mM PB, pH7.5, 150mM NaCl buffer. |
M.W. |
12, 774 Da |
Product Line |
Cytokine, Chemokines & Growth Factors |
Reconstitution |
It is recommended to reconstitute the lyophilized, Recombinant Human Interleukin 15 (IL-15), in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
Storage |
Lyophilized, recombinant human Interleukin 15 (IL-15), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin 15 (IL-15) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles. |
Manufacturers Category |
Proteins |
Manufacturers Product Line |
Cytokines |
Product Origin |
USA |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
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