IL-2, Human 

In vitro, studies performed on human cell lines demonstrate the immunoregulatory properties of Interleukin-2 (IL-2) including: 1) enhancement of lymphocyte mitogenesis and stimulation of long-term growth of human Interleukin-2 (IL-2) dependent edll lines, 2) enhancement of lymphocyte cytotoxicity, 3) induction of killer cell (lymphokine-activated (LAK) and natural (NK)) activity, 4) induction of interferon-gamma production. Recombinant human Interleukin-2 (IL-2), is a highly purified protein with a molecular weight of approximately 15, 300 Da. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered, E. coli, strain containing an analog of the human Human Interleukin-2 (IL-2) gene. Genetic engineering techniques were used to modify the human Interleukin-2 (IL-2) gene, and the resulting expression clone encodes a modified human Interleukin-2 (IL-2). This recombinant form differs from native Interleukin-2 (IL-2) in following ways: 1) it is not glycosylated, 2) the molecule has no N-terminal alanine, 3) the molecule has serine substituted for cysteine at amino acid position 125, 4) the aggregation state of molecule is likely to be different from that of native Interleukin-2 (IL-2).

The protein was lyophilized after extensive dialysis against 50mM Phosphate buffer, pH7.5, 5% Mannitol buffer.

Cytokine, Chemokines & Growth Factors

Lyophilized, recombinant human Interleukin-2 (IL-2), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin-2 (IL-2) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.

Cytokines