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IL-2, Human

IL-2, Human

Item no.	Z00368-1
Manufacturer	GenScript
Amount	1 mg
Category	Oncology Food Safety Neuroscience Neural Lineage Markers Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Cell Status Apoptosis Proliferation Toxicity & Cytotoxicity Contaminants Metals By Organ Breast Cancer Heart & Blood Cancer
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	Greater than 98% as determined by the following methods: (a) SEC-HPLC analysis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis
Citations	Yu J, et al . Interleukin-2 reconstitutes defective human immunodeficiency virus (HIV), and cytomegalovirus (CMV) specific CD8+ T cell proliferation in HIV infection. J. Med. Virol. Sep 2006, 78 (9): 1147-1157. Perchonock CE, et al . Negative Regulation of Interleukin-2 and p38 Mitogen-Activated Protein Kinase during T-Cell Activation by the Adaptor ALX. Mol. Cell. Biol. Aug 2006, 26 (16): 6005-6015. Gujar SA, Michalak TI. Characterization of bioactive recombinant woodchuck interleukin-2 amplified by RLM-RACE and produced in eukaryotic expression system. Vet. Immunol. Immunopathol. Aug 2006, 112 (3-4): 183-198.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	In vitro, studies performed on human cell lines demonstrate the immunoregulatory properties of Interleukin-2 (IL-2) including: 1) enhancement of lymphocyte mitogenesis and stimulation of long-term growth of human Interleukin-2 (IL-2) dependent edll lines, 2) enhancement of lymphocyte cytotoxicity, 3) induction of killer cell (lymphokine-activated (LAK) and natural (NK)) activity, 4) induction of interferon-gamma production. Recombinant human Interleukin-2 (IL-2), is a highly purified protein with a molecular weight of approximately 15,300 Da. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered, E. coli, strain containing an analog of the human Human Interleukin-2 (IL-2) gene. Genetic engineering techniques were used to modify the human Interleukin-2 (IL-2) gene, and the resulting expression clone encodes a modified human Interleukin-2 (IL-2). This recombinant form differs from native Interleukin-2 (IL-2) in following ways: 1) it is not glycosylated, 2) the molecule has no N-terminal alanine, 3) the molecule has serine substituted for cysteine at amino acid position 125, 4) the aggregation state of molecule is likely to be different from that of native Interleukin-2 (IL-2).</td></tr><tr><th>M.W.</th><td colspan="7"> 15,300 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 98% as determined by the following methods: (a) SEC-HPLC analysis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (1 EU/ug) of recombinant human Interleukin-2 (IL-2) as determined by LAL test.</td></tr><tr><th>Specific Activity</th><td colspan="7"> The specific activity as determined and was found to be greater than 1.0×107 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, recombinant human Interleukin-2 (IL-2), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin-2 (IL-2) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 50mM Phosphate buffer, pH7.5, 5% Mannitol buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized recombinant human Interleukin-2 (IL-2) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Reference</th><td colspan="7">Yu J, et al . Interleukin-2 reconstitutes defective human immunodeficiency virus (HIV), and cytomegalovirus (CMV) specific CD8+ T cell proliferation in HIV infection. J. Med. Virol. Sep 2006, 78 (9): 1147-1157. Perchonock CE, et al . Negative Regulation of Interleukin-2 and p38 Mitogen-Activated Protein Kinase during T-Cell Activation by the Adaptor ALX. Mol. Cell. Biol. Aug 2006, 26 (16): 6005-6015. Gujar SA, Michalak TI. Characterization of bioactive recombinant woodchuck interleukin-2 amplified by RLM-RACE and produced in eukaryotic expression system. Vet. Immunol. Immunopathol. Aug 2006, 112 (3-4): 183-198.</td></tr>
Similar products	Interleukin-2
Available
Specificity	The specific activity as determined and was found to be greater than 1.0x107 IU/mg.
Specificity	The specific activity as determined and was found to be greater than 1.0x107 IU/mg.
Description	In vitro, studies performed on human cell lines demonstrate the immunoregulatory properties of Interleukin-2 (IL-2) including: 1) enhancement of lymphocyte mitogenesis and stimulation of long-term growth of human Interleukin-2 (IL-2) dependent edll lines, 2) enhancement of lymphocyte cytotoxicity, 3) induction of killer cell (lymphokine-activated (LAK) and natural (NK)) activity, 4) induction of interferon-gamma production. Recombinant human Interleukin-2 (IL-2), is a highly purified protein with a molecular weight of approximately 15, 300 Da. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered, E. coli, strain containing an analog of the human Human Interleukin-2 (IL-2) gene. Genetic engineering techniques were used to modify the human Interleukin-2 (IL-2) gene, and the resulting expression clone encodes a modified human Interleukin-2 (IL-2). This recombinant form differs from native Interleukin-2 (IL-2) in following ways: 1) it is not glycosylated, 2) the molecule has no N-terminal alanine, 3) the molecule has serine substituted for cysteine at amino acid position 125, 4) the aggregation state of molecule is likely to be different from that of native Interleukin-2 (IL-2).
Endotoxin Level	Less than 0.1 ng/ug (1 EU/ug) of recombinant human Interleukin-2 (IL-2) as determined by LAL test.
Formulation	The protein was lyophilized after extensive dialysis against 50mM Phosphate buffer, pH7.5, 5% Mannitol buffer.
M.W.	15, 300 Da
Product Line	Cytokine, Chemokines & Growth Factors
Reconstitution	It is recommended to reconstitute the lyophilized recombinant human Interleukin-2 (IL-2) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage	Lyophilized, recombinant human Interleukin-2 (IL-2), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin-2 (IL-2) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.
Manufacturers Category	Proteins
Manufacturers Product Line	Cytokines
Product Origin	USA

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Amount: 1 mg

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