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CHAPS Lysis and Immunoprecipitation Buffer (CIB-1)

CHAPS Lysis and Immunoprecipitation Buffer (CIB-1)

Item no.	CIB-1-60
Manufacturer	Fivephoton
Amount	60 ml
Category	Oncology Metabolism Obesity Reagents & Chemicals Biochemicals Buffers By Organ Lung Cancer Skin Cancer
Type	Buffer
Specific against	other
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12161700
Available
Highlights	Strong cell lysis and protein extraction capacity while preserving protein conformation and protein-protein interaction for co-immunoprecipitation (Co-IP) analysis. Ingredients: 0.5% CHAPS, HEPES, NaCl Effective as a stand alone reagent for cell lysis and protein extraction as well as for immunoprecipitation. Contains a non-amine buffer (HEPES) enabling cross-linking with NHS-ester derivatives. Compatible with Bradford, Lowry and BCA protein assays. 60 ml volume: Suitable for two hundred 10-cm culture plates, or six hundred wells in 6-well dishes. 120 ml volume is also available. Storage: 4C. Ships at ambient temperature.
References	Darby PJ, Kwan CY, Daniel EE. 2000. Caveolae from canine airway smooth muscle contain the necessary components for a role in Ca(2+) handling. Am J Physiol Lung Cell Mol Physiol 279: L1226-35. Farmery MR, Allen S, Allen AJ, Bulleid NJ. 2000. The role of ERp57 in disulfide bond formation during the assembly of major histocompatibility complex class I in a synchronized semipermeabilized cell translation system. J Biol Chem 275: 14933-8. Ora A, Helenius A. 1995. Calnexin fails to associate with substrate proteins in glucosidase-deficient cell lines. J Biol Chem 270: 26060-2. Pieren M, Gali C, et. al. 2005. The use of calnexin and calreticulin in cellular and viral glycoproteins. J Biol Chem 280: 28265-71. Zhu X, Peng J, Chen D, Liu X, Ye L, et al. 2005. Calnexin and ERp57 facilitate the assembly of the neonatal Fc. Journal of Immunology 175: 967-76.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 60 ml

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Delivery expected until 6/5/2025

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