Leuco-Malachite Green-NHS Ester (HPT1103)

Leuco-Malachite Green NHS Ester is a labeling reagent precursor of malachite green. After leuco-malachite green nhs ester is first linked to a macromolecule through reaction with a primary amino group, and then oxidized with reagents like DDQ, a malachite green modification is generated. The leuco-malachite green modification can be used as a precursor material for any malachite green derivative that involves a primary amino reaction. See below for oxidation and conversion methods.
Excess DDQ (2, 3-dichloro-5, 6-dicyano quinone) is dissolved in DMSO or DMF, and added to the solution of leuco malachite green - target conjugate (i.e oligonucleotide previously modified with leuco-malachite green nhs ester). The leuco-malachite green modification group is immediately oxidized to malachite green, which results in an intense green-blue coloration. The resulting malachite green - target conjugate can be purified by an appropriate method.