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FD Rapid TimmStain™ Kit

FD Rapid TimmStain™ Kit

Item no.	PK701
Manufacturer	FD NEUROTECHNOLOGIES
Amount	1 kit
Category	Neuroscience Other Assays & Kits Other Kits
Type	Kit
Specific against	other
Citations	Haug FMS. (1973) Heavy metals in the brain: a light microscope study of the rat with Timm’s sulphide silver method. Methodological considerations and cytological and regional staining patterns. Springer-Verlag Berlin Heidelberg New York. Sloviter RS. (1982) A simplified Timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain. Brain Res. Bulletin. 8:771-4.
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Manufacturer - Category	Staining kits
Description	Timm’ s sulphide silver method has so far been considered a very sensitive technique for demonstrating metal ions, in particular zinc in the central nervous system. The principle of this technique is that metals in the tissue can be transformed histochemically to metal sulphides. Subsequently, metal sulphides catalyze the reduction of silver ions by a reducing agent to metallic grains that are visible under a light or electron microscope. Studies using this technique have provided a better understanding of not only the localization and distribution of zinc but also its possible function in the brain¹. However, the reliability and complexity of Timm’ s sulphide silver staining have been major obstacles to the widespread application of this technique. FD Rapid TimmStain™ Kit is designed based on the methods described by Haug¹ and Sloviter². The reagents and procedure of the FD Rapid TimmStain™ Kit have been optimized to achieve a high degree of both specificity and sensitivity for the demonstration of metal ions, especially zinc in the brain of experimental animals. The kit can be used with both frozen and paraffin sections.
Materials required but not included	Double distilled or deionized water 10% neutral buffered formalin 0.1 M phosphate buffer (pH 7.4) Histological supplies and equipment: Microscope slides Coverslips Staining jars Xylenes or xylene substitutes Resinous mounting medium (e.g. Permount^®) A light microscope

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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PK701-datasheet.pdf

PK701-safety-datasheet.pdf