« Back
Home /
High Speed Plasmid Midi Kit (25prep), Ion Exchange

High Speed Plasmid Midi Kit (25prep), Ion Exchange

Item no.	FAPDE002-SP2
Manufacturer	Favorgen Biotech
Amount	25 preps
Category	Other Assays & Kits Extraction Kits
Type	Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Additional Requirement To Be Provided By User	1. Centrifuge rotor and bottle for up to 150 ml cell culture and capable of speed at 4, 5000ca.6, 000 x g 2. 50 ml centrifuge 3. Isopropanol 4. 70% ethanol 5. TE buffer, PH7.0 or ddH2O, PH7.0
Appropriate Plasmid Size	2kp ca. 50 kb
Binding Capacity	up to 800 ug of DNA
Important Notes	1. Store RNase A at -20 C upon recipit of kit. 2. Add 0.5 ml of PM1 Buffer to a RNase A tube, vortex the tube to mix well. Transfer the total RNase A mixture back to the PM1 bottle, mix well by vortexing and store the PM1 buffer at 4 C. 3. If precipitates have formed in PM2 Buffer, warm the buffer in 37C waterbath to dissolve preciptates. 4. Pre-chill PM3 Buffer at 4 C before starting.
Lysate Clarification	Filtration
Operation Time	plasmid size <= 50 kb: 45 min plasmid size > 50 kb: 90 min
PM Mini Column (Ion-Exchange Resin Column)	up to 150 ml of bacteria culture for low-copy number plasmid
Stored At	Store at room temperature (15ca. 25 C) for 2 year. Except RNAse A Powder, store at -20C.
Technology	Anion-exchange chromatography (gravity-flow column)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 25 preps

available

Delivery expected until 10/18/2024

Express Delivery: Save 24 days

Compare

Add to wishlist

Recommend by mail