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ELISA Kit for Elastase 3B (ELA3B)

ELISA Kit for Elastase 3B (ELA3B)

Manufacturer	Cloud-Clone
Category	Oncology ELISA Cardiovascular Research Atherosclerosis ELISA & Immunoassays ELISA Kits Immunoassay By Organ Pancreatic Cancer
Type	Elisa-Kit
Specific against	Rat
Amount	48T
Item no.	SEE212Ra-48T
eClass 6.1	32160605
eClass 9.0	32160605
Available
Alias	CELA3B, Chymotrypsin Like Elastase Family Member 3B, Protease E, Fecal Elastase, Elastase 1, Cholesterol-Binding Pancreatic Protease, Pancreatic Endopeptidase E
Quantity options
Detection range	0.156-10ng/mL
Organism species	Rattus norvegicus (Rat)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.054ng/mL
Sample type	serum, plasma, tissue homogenates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Elastase 3B (ELA3B). No significant cross-reactivity or interference between Elastase 3B (ELA3B) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50µ L Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3B (ELA3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3B (ELA3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3B (ELA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 3B (ELA3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area	Enzyme & Kinase;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.