Item no. |
SED539Hu-96T |
Manufacturer |
Cloud-Clone
|
Amount |
96T |
Category |
|
Type |
Elisa |
Specific against |
Human |
Sensitivity |
The minimum detectable dose of this kit is typically less than 0.054ng/mL |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
VDD1, PDDR; CYP1, P450c1; 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase; Cytochrome P450 Family 27 Subfamily B Polypeptide 1; Calcidiol 1-monooxygenase; VD3 1A hydroxylase |
Available |
|
Specificity |
This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 27B1 (CYP27B1). No significant cross-reactivity or interference between Cytochrome P450 27B1 (CYP27B1) and analogues was observed. |
Manufacturers Category |
ELISA Kit |
Application |
Enzyme-linked immunosorbent assay for Antigen Detection. |
Sample type |
tissue homogenates, cell lysates and other biological fluids |
Assay length |
3h |
Method |
Double-antibody Sandwich |
Precision |
Intra-assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Assay procedure summary |
1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50uL Stop Solution. Read at 450nm immediately. |
Test principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27B1 (CYP27B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27B1 (CYP27B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27B1 (CYP27B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 27B1 (CYP27B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Item Name |
Cytochrome P450 27B1 |
Manufacturers Research Field |
Metabolic pathway; |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.